“Variable induction of experimental abdominal aortic aneurysms with different preparations of porcine pancreatic elastase”

Sodium dodecylsulfate-polyacrylamide gel electrophoresis of “old” and “new” preparations of Sigma E-1250 type I porcine pancreatic elastase, run under nondenaturing conditions and stained for total protein (2 μL per lane). Elastase is expected to migrate at approximately 30 kD (*). Several of the proteins removed during the “enhanced” preparation of E-1250 are indicated by arrows. Although the “old” preparation is effective in the induction of experimental abdominal aortic aneurysms, the “new” preparation is not. Mr, Molecular weight markers; AAA, abdominal aortic aneurysms.