Journal of Vascular Surgery
Volume 50, Issue 6 , Pages 1452-1458 , December 2009

VCAM-1 siRNA reduces neointimal formation after surgical mechanical injury of the rat carotid artery

  • Yanming Qu, MD

      Affiliations

    • Department of Neurosurgery, Capital Medical University Affiliated Fu Xing Hospital, Beijing PR China
    • ,
  • Xiangen Shi, MD

      Affiliations

    • Department of Neurosurgery, Capital Medical University Affiliated Fu Xing Hospital, Beijing PR China
    • ,
  • Hongwei Zhang, MD

      Affiliations

    • Department of Neurosurgery, Brain Sciences Institute of Beijing, Beijing PR China
    • ,
  • Wei Sun, MD

      Affiliations

    • Department of Neurosurgery, Capital Medical University Affiliated Fu Xing Hospital, Beijing PR China
    • ,
  • Song Han, MD

      Affiliations

    • Department of Neurobiology and Beijing Institute for Neuroscience, Capital Medical University, Beijing PR China
    • ,
  • Chunjiang Yu, MD

      Affiliations

    • Department of Neurosurgery, Capital Medical University Affiliated Fu Xing Hospital, Beijing PR China
    • Corresponding Author InformationChunjiang Yu, MD, Department of Neurosurgery, Capital Medical University Affiliated Fu Xing Hospital, No. 20 Fu Xing Men Wai Street, Beijing 100038, China
    • ,
  • Junfa Li, MD, PhD

      Affiliations

    • Department of Neurobiology and Beijing Institute for Neuroscience, Capital Medical University, Beijing PR China
    • Corresponding Author InformationReprint requests: Junfa Li, MD, PhD, Department of Neurobiology and Beijing Institute for Neuroscience, Capital Medical University, No. 10 You An Men Wai Xi Tou Tiao, Beijing 100069, China

Received 17 May 2009 ,Accepted 15 August 2009.

  • Image Result

    Lentivirus-based VCAM-1 siRNA transfection in carotid arteries of rats following CSMDE. Representative images showing no detectable green fluorescence in normal (CON, A) animals but pronounced fluores

    Lentivirus-based VCAM-1 siRNA transfection in carotid arteries of rats following CSMDE. Representative images showing no detectable green fluorescence in normal (CON, A) animals but pronounced fluorescence in the neointima of carotid arteries after transfection with lentiviral constructs co-expressing GFP (TRNAS, B); C, Western blotting of carotid arterial homogenates showing the expression of GFP (27 kDa) and β-actin (42 kDa) in CON and TRANS animals; D, Quantitative analysis of Western blot signals shows that GFP expression levels increased significantly in carotid arterial homogenates from TRANS animals (*P < .05 vs CON, n = 6 per group).

  • Image Result
    siRNA blocks CSMDE-induced VCAM-1 upregulation in rat carotid arteries. A, Representative Western blot showing VCAM-1 (110 kDa) protein expression levels in carotid arteries of rats in the SHAM, CSMDE

    siRNA blocks CSMDE-induced VCAM-1 upregulation in rat carotid arteries. A, Representative Western blot showing VCAM-1 (110 kDa) protein expression levels in carotid arteries of rats in the SHAM, CSMDE, CSMDE+CON and CSMDE+RNAi groups. B, Quantitative analysis reveals significant upregulation of VCAM-1 protein expression following CSMDE and CSMDE+CON (*P < .05 vs SHAM). VCAM-1 siRNA significantly reduced VCAM-1 protein expression compared with CSMDE+CON (#P < .05). SHAM, Sham operation; CSMDE, carotid surgical mechanical de-endothelialization; CSMDE+CON, CSMDE+scrambled siRNA; CSMDE+RNAi, CSMDE+VCAM-1 siRNA; n = 10 per group.

  • Image Result
    Doppler ultrasonographic measurement of rat carotid arteries. A, Representative images showing carotid artery linear diameter and blood velocity measurements; left and right panels respectively show t

    Doppler ultrasonographic measurement of rat carotid arteries. A, Representative images showing carotid artery linear diameter and blood velocity measurements; left and right panels respectively show the blood vessel lumens and blood flow spectrograms at the sites of greatest narrowing (arrowheads indicate the points of stenosis). B, Quantitative analysis showing that both arterial linear diameter and blood velocity are significantly decreased in the CSMDE and CSMDE+CON groups (*P < .05 vs SHAM). Treatment of VCAM-1 siRNA transfection (CSMDE+RNAi) resulted in a significant increase in linear diameter and blood velocity (#P < .05 vs CSMDE+CON). n = 10 per group.

  • Image Result
    Inhibition of carotid artery neointimal hyperplasia by local VCAM-1 siRNA expression in rats following CSMDE. Representative light microscope images of carotid artery cross-sections from SHAM, CSMDE,

    Inhibition of carotid artery neointimal hyperplasia by local VCAM-1 siRNA expression in rats following CSMDE. Representative light microscope images of carotid artery cross-sections from SHAM, CSMDE, CSMDE+CON and CSMDE+RNAi animals, scale bar = 200 μm.

 Supported by the following grants: National Natural Science Foundation of China (30571902, 30670782, and 30871219), Beijing Natural Science Foundation (5072008), Key Scientific Developing Programs of Beijing Municipal Commission of Education (KZ200810025012), Beijing Municipal Programs for Hundred-Thousand-Ten Thousand Excellent Talents of New Century (Li J), and Funding Projects for Academic Human Resources Development in Institutions of Higher Learning under the Jurisdiction of Beijing Municipality (PHR200906116).

 Competition of interest: none.

 The editors and reviewers of this article have no relevant financial relationships to disclose per the JVS policy that requires reviewers to decline review of any manuscript for which they may have a competition of interest.

PII: S0741-5214(09)01696-6

doi: 10.1016/j.jvs.2009.08.050

Journal of Vascular Surgery
Volume 50, Issue 6 , Pages 1452-1458 , December 2009

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