Journal of Vascular Surgery
Volume 50, Issue 2 , Pages 388-395 , August 2009

Mast cells associate with neovessels in the media and adventitia of abdominal aortic aneurysms

  • Mikko I. Mäyränpää, MD, PhD

      Affiliations

    • Wihuri Research Institute, Helsinki, Finland
    • Department of Forensic Medicine, University of Helsinki, Helsinki, Finland
    • Corresponding Author InformationReprint requests: Mikko Mäyränpää, MD, PhD, Wihuri Research Institute, Kalliolinnantie 4, 00140 Helsinki, Finland
    • ,
  • Julia A. Trosien, MSc

      Affiliations

    • Wihuri Research Institute, Helsinki, Finland
    • ,
  • Vincent Fontaine, MD, PhD

      Affiliations

    • Atherosclerosis Research Unit, Center for Molecular Medicine, Department of Medicine, Karolinska Institute, Stockholm, Sweden
    • ,
  • Maggie Folkesson, MSc

      Affiliations

    • Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, Sweden
    • ,
  • Monsur Kazi, MD, PhD

      Affiliations

    • Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, Sweden
    • Atherosclerosis Research Unit, Center for Molecular Medicine, Department of Medicine, Karolinska Institute, Stockholm, Sweden
    • ,
  • Per Eriksson, PhD

      Affiliations

    • Atherosclerosis Research Unit, Center for Molecular Medicine, Department of Medicine, Karolinska Institute, Stockholm, Sweden
    • ,
  • Jesper Swedenborg, MD, PhD

      Affiliations

    • Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, Sweden
    • ,
  • Ulf Hedin, MD, PhD

      Affiliations

    • Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, Sweden

Received 1 December 2008 ,Accepted 14 March 2009.

Title About Type File Size
Supplementary Fig I

Representative double immunofluorescent photomicrographs showing stem cell factor (green) positive endothelial cells and tryptase (red) positive mast cells (arrows). In a normal aortic (A) wall stem cell factor is also expressed in some intimal cells, including mast cells, whereas in aneurysms stem cell factor was mostly confined to the endothelium of neovessels (B). Scale bars 50 μm. Background staining DAPI.

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Supplementary Fig II

Representative staining showing platelets stained for CD42b (DAB, brown) in a TH AAA wall (A) and an NTH AAA (B). The observed lack of endothelial cell staining in NTH AAA specimens was supported by the finding of thin luminal deposit of platelets (luminal arrow head). CD42b staining was also observed in the intima of NTH AAA specimens (arrowheads). Scale bars 100 μm. Counterstained with Mayer's hematoxylin.

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Supplementary Table Other 0 MB

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 Supported by grants from the Finnish Foundation for Cardiovascular Research (M.I.M.), the Swedish Research Council (12233, 12660), and the Swedish Heart-Lung foundation and the EC Seventh Framework Programme (FAD-200647) and supported by the Marie Curie Early Stage Research Training Fellowship of the European Community's Sixth Framework Programme, contract number 504926 (J.A.T.).

 Competition of interest: none.

 Additional material for this article may be found online at www.jvascsurg.org.

PII: S0741-5214(09)00798-8

doi: 10.1016/j.jvs.2009.03.055

Journal of Vascular Surgery
Volume 50, Issue 2 , Pages 388-395 , August 2009

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