Fibrin monomer and fibrinopeptide B act additively to increase DNA synthesis in smooth muscle cells cultured from human saphenous vein

Fibrinogen and fibrin stimulation of DNA synthesis in SMCs cultured from human saphenous vein. Cells were cultured on uncoated plastic, and protein was added for 24 hours before measurement of DNA synthesis (BrdU incorporation). Results (mean ± SD) are reported as percentage DNA synthesis in comparison with controls with no added protein.

Stimulation of DNA synthesis in SMCs by fibrinogen and fibrin monomer for cells on fibronectin. Cells were cultured on fibronectin-coated plastic, and protein was added for 24 hours before measurement of DNA synthesis (BrdU incorporation). Results (mean ± SD) are reported as percentage DNA synthesis in comparison with controls with no added protein. Results for cells cultured on type I collagen polymer were almost identical, with superimposed curves.

Inhibition of the fibrinogen-mediated stimulation of DNA synthesis for SMCs cultured on monomeric collagen. Cells have been cultured on plastic coated with collagen monomer, and protein was added for 24 hours before measurement of DNA synthesis (BrdU uptake). Amiloride was used at 10 μmol/L and PPACK at 1 μmol/L. Results (mean ± SD) are reported as percentage DNA synthesis in comparison with controls with no added protein.

The binding of labeled fibrinopeptide B to SMCs cultured from human saphenous vein. Iodinated fibrinopeptide B-tyr was incubated at 4°C with confluent monolayers of SMCs cultured on uncoated plastic. Results show saturable binding (mean ± SD for 5-6 wells). Scatchard plot (inset ) suggests presence of a single, high-affinity, binding site, with an apparent K_d of 0.6 μmol/L calculated from binding curve. B (tyr) , Tyrosine derivative of fibrinopeptide B.