The S130K fibroblast growth factor–1 mutant induces heparin-independent proliferation and is resistant to thrombin degradation in fibrin glue

Molecular degradation of FGF-1 and S130K by thrombin. FGF-1 and S130K were incubated with thrombin 6 U for the specified times (x-axis) and then analyzed by SDS/PAGE. Molecular markers (y-axis) are shown on the left for both gels. FGF-1 and S130K are 17 kd molecules. Both molecules are susceptible to thrombin degradation, but S130K is relatively more resistant than FGF-1, as demonstrated by the delayed appearance and diminished intensity of low molecular weight fragments, suggesting that the mutation offers some protection from thrombin degradation.